Illumination techniques and its applications

The right illumination is key in stereo microscopy to contrast the surface of the specimen and therefore to allow an informative investigation and optical documentation. But to select the right illumination might be a challenge. Various lighting options with different characteristics are available. They will strongly influence if you are able to investigate your specimen in a proper way. Learn more about the options to make sure that you choose the best product according to your requirements.


Lighting Options on a stereo microscope

Incident Light:

Incident light or reflected light is used for non-transparent specimens. With different lighting techniques, the sample can be illuminated from various angles which will result in different shadow effects for contrasting. The choice depends on the characteristic of the specimen. Typical light options are:
  • Ring Lights
  • Spot Light / Gooseneck / Flexible Light Guide
  • Line Light

Transmitted Light:

Transmitted Light is used for transparent specimen to illuminate the sample from the bottom. The equipment for transmitted light is called Back Light.


Optical Accessories:

Optical Accessories are used to influence the light quality. Typical options are polarization filters, diffusors, and colour filters. We offer a broad variety in all our 3 series:
KL |
| VisiLED


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Example Ring Light

Bright Field Ring Light

Ring Lights are attached on the objective. If the ring light is fully illuminated (Full Circle Mode), the sample is illuminated homogeneously and free from shadows from all sides. In addition, some of our LED ring lights have Segment Modes which allows for only parts of the ring light to be illuminated and rotated around the sample. This allows surface topography to be highlighted and can avoid reflections on cylindrical samples.

Typical application areas:
Full circle mode:
Applications where homogeneous illumination is most important, e.g. for stiching of images
Segment mode:
Flat surfaces like leather or shiny samples like a light bulb where reflections shall be avoided

Without segment mode:
KL Ring Lights | EasyLED Ring Light
With segment mode:
EasyLED Ring Light Plus | VisiLED Ring Lights
Samples illuminated with bright field and UV light

UV Ring Light

UV light is generally used in stereomicroscopy to contrast the sample with fluorescent light. It is below the visible wavelengths, but excites many materials to emit visible fluorescence.

Typical Application areas:
Examination of materials with fluorescent markers or autofluorescence. Typical application fields include forensic examination of documents, analysis of cracks, paints, varnishes etc.

VisiLED UV Ring Light

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