Next-generation process control
Kristina Gruber discusses the key benefits of utilizing newly-developed PAT components in Raman spectroscopy.
What is Raman spectroscopy?Raman spectroscopy is an analytical technique used in life sciences to derive structural information for the purpose of identifying molecules. Samples are illuminated with laser light, which triggers molecular vibrations that can be used to determine chemical composition and other vital material properties, such as measuring concentrations.
Why is Raman spectroscopy useful?Due to the light-based nature of the process, Raman spectroscopy is a non-destructive and non-contact process. Required handling and preparation of samples is minimal, and analysis can be conducted using a number of approaches that includes studying individual input materials, mid-process composition, and final product checks.
Analysis time is fast and supports automation and increased capability for process control. The high molecular specificity of Raman scattering enables the analysis of non-homogenous compositions, and the process is proven hands-on in multi-use or laboratory bioreactors using existing standardized ports.
Advantages of ViewPort® components
Connected to standardized ports
Sterilizable with bioreactor
Interface for sensors or spectrometers
Real-time monitoring through optical window
Exchangeable any time at running cultivation providing sterile integrity
Continuing real-time monitoring
How can ViewPort® technology be integrated for Raman spectroscopy?
ViewPort® components reduce the number of components that require sterilization, while an easy change-out process eliminates the need for instrument redundancy and the risk of contamination. Optical instruments can also be shared between bioreactors, and ports can be shared with additional optical tools without issue. Single-use bioreactor processes can be validated using existing equipment using a bellows port, and ports can be implemented in single-use bioreactor bags for future innovation in process analysis.
October 29th, 2022
Manager R&D Biotech and Life Sciences