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Uncoated slides

NEXTERION® Glass B and Glass D

SCHOTT provides two different types of uncoated glass slides, NEXTERION® Glass B (BOROFLOAT® 33) and D (D263T), for customers looking to apply their own functional coatings. Both glass materials are borosilicate glasses with high chemical resistance, excellent transmission, low fluorescence, and exceptional flatness and were specially selected by SCHOTT Microarray Solutions as the optimal glass types for microarray related applications. The naturally pristine glass surfaces can be used without any additional polishing steps and all the slides are laser cut to minimize particle contamination.

NEXTERION® Glass B

Autofluorescence NEXTERION® Glass D and Glass B vs. competitors NEXTERION® Glass B is a highly chemically resistant borosilicate glass that is produced by melting the purest raw materials. The microfloat process is used for manufacturing this glass type, resulting in a pristine, fire-polished surface that can be used without any additional polishing. This process allows the production of glass substrates with tight geometric properties. In addition, the fluorescence is particularly low in the range of Cy3™ and Cy5™ emission wavelengths (570 nm and 670 nm), making NEXTERION® glass slides the perfect substrate solution for microarray applications.

NEXTERION® Glass D

NEXTERION® Glass D is a high quality borosilicate glass produced by melting the purest raw materials. It is manufactured by a special down-draw production process that results in fire-polished surfaces that can be used without any additional processing. The NEXTERION® Glass D production process allows SCHOTT to offer glass substrates with extremely tight geometric properties. In addition, the fluorescence is particularly low in the range of Cy3™ and Cy5™ emission wavelengths (570 nm and 670 nm).

NEXTERION® Glass B and D specification

NEXTERION® uncoated slides manufactured to the following specifications: 25 mm x 75.6 mm ± 0.1 mm Thickness 1.0 mm ± 0.05 mm Plain cut edges, by using the precise laser cutting process

Detailed information about Glass B flatness

The slides are available with or without a barcode (code 128). Customized features for particular applications are available upon request and include slide orientation marks, non-standard barcodes, customized barcode numbering, company logos, etc. Please enquire for further information.

Laser cutting

Properties NEXTERION® Glass B (BOROFLOAT® 33) NEXTERION® Glass D (D263T) Mechanical • Density ρ in g/cm3 2.2 2.51 Optical • Refractive indices ne (λ = 546.1 nm) nd (λ = 587.6 nm) • Dispersion (nF – nC) • Abbe value (νe) • Luminous transmittance (τvD65) (Glass thickness 1.1 mm) • Stress optical coefficient C in 1.02 x 10-12 m²/N 1.47311 1.47140 71.9 x 10–4 65.41 92.7% 4.0 1.5255 1.5231 96.0 x 10–4 55 91.7%±0.3% 3.4 Chemical • Hydrolytic resistance(ISO 719/DIN 12 111) • Acid resistance(ISO 1776/DIN 12 116) • Alkali resistance(ISO 695/DIN 52 322) HGB 1 1 A 2 HGB 1 2 A 2 Thermal • Linear thermal coefficient of expansion α (20 – 300 °C/68 – 572 °F) • Transformation temperature Tg 3.25 x 10-6/K 525°C 7.2 x 10-6/K 557°C

The properties detailed above were calculated using the very latest standards and measuring methods. SCHOTT reserves the right to change the data in keeping with the latest technical standards. Numerical values stated without tolerances are reference values of an average production quality. All data is intended to be used as a guideline, unless otherwise stated. Please contact us if you require further information.

Three levels of cleanliness

Description Cleaning process Packaging External Packaging Comments Uncleaned Deionised water in a conventional washing machine with brushes. “Grey” room conditions Plastic storage boxes “Grey” room conditions None Recommended if a thorough cleaning procedure is used prior to further processing. Ultrasonically cleaned Automated ultrasonic cleaning line using multiple wash steps of varying pH’s, rinse steps and final drying stage. Class 100 clean room Plastic storage boxes Class 100 clean room None Recommended if a simple cleaning procedure is used prior to further processing. Clean room cleaned Automated ultrasonic cleaning line using multiple wash steps of varying pH’s, rinse steps and final drying stage. Class 100 clean room Plastic storage boxes Class 100 clean room Sealed in air-tight laminated foil pouches under inert Argon atmosphere Class 100 clean room Glass may be used without any cleaning steps.

For more information please download the presentation NEXTERION^® class cleaning

Automated ultrasonic cleaning of slides

Frequently asked questions

What is the difference between SCHOTT NEXTERION® Glass B and D? What is the tensile strength of NEXTERION® Glass B and D, how do these values compare to soda lime glass? How does the hardness of Glass B and D compare to Soda lime glass? Has BOROFLOAT® 33 / NEXTERION® Glass B been tested for hydrolytic resistance in accordance with the European Pharmacopeia EP 6 (2010) Chapter 3.2.1 and United States Pharmacopeia USP 32? What is the average surface micro roughness (Ra) of Glass B (BOROFLOAT® 33) and D (D263)? Are the properties of the two Glass D (D263) surfaces identical? NEXTERION® Glass B (BOROFLOAT® 33) is produced by the microfloat process, resulting in a "fire-polished" upper surface, and a lower surface that has been in contact with molten tin. Is it possible to tell the difference between the two surfaces? What is the "flatness" of Glass B (BOROFLOAT® 33)? Can SCHOTT offer any advice on the best method of bonding Glass D (D263) to Polydimethylsiloxane (PDMS)? In the NEXTERION® Glass B specification, a number of spot type defects are listed under "Surface specification". What do these terms mean? Can you certify that NEXTERION® Glass B / BOROFLOAT 33 does not contain any toxic heavy metals? We are using NEXTERION® Glass B / BOROFLOAT® 33 in a diagnostic product / medical device, can you certify that the glass does not contain any animal derived material? Does SCHOTT offer alternative glass types for biological applications? What is the difference between the three levels of cleanliness of the SCHOTT NEXTERION® uncoated slides? What is the refractive index of the glass used to manufacture the NEXTERION® Glass B slides? What is the refractive index of the glass used to manufacture the NEXTERION® Glass D slides? Which excitation and emission wavelengths has the Glass B and D been tested with for auto-fluorescence? What is the transmission / absorbance spectrum of Glass B (BOROFLOAT® 33)

Download pdf file NEXTERION^® uncoated slides

Product Specifications

Specification NEXTERION^® Glass B cleanroom cleanedSpecification NEXTERION^® Glass B ultrasonically cleanedSpecification NEXTERION^® Glass B uncleanedSpecification NEXTERION^® Glass D cleanroom cleanedSpecification NEXTERION^® Glass D ultrasonically cleanedSpecification NEXTERION^® Glass D uncleaned