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Spotting
Nexterion® spotting buffers
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SCHOTT Microarray Solutions offers a range of optimized spotting buffers
that are compatible with the Nexterion® slide surfaces. Although many users
printing DNA and protein microarrays opt to work with commonly used
spotting buffers, such as 3X SSC and 50 % DMSO (most of which work well
with the Nexterion® coated substrates), SCHOTT has received many requests
for fully optimized spotting buffers to significantly reduce the time and effort
typically associated with spotting buffer validation. SCHOTT not only offers
a standard spotting buffer that works well with most slide surfaces, but we
also now offer new low evaporation spotting buffers to solve one of the most
commonly observed problems associated with using aqueous spotting buffers.
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| The choice of the spotting buffer plays an important role in binding
capacity and spot morphology. Therefore it is extremely important
to fully test slides with the appropriate buffer. |
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| Spot morphology and signal intensity strongly depend on following
parameters |
| • Composition of spotting solution |
| • Compatibility of spotting buffer and slide surface chemistry |
| • Spot density/pitch |
| • Nature, concentration, and purity of probe molecule |
| • Spotting technology |
| • Environmental conditions |
| • Immobilization procedures |
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Nexterion® Spot
(order code: 1066029)
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DNA spotting buffer
Nexterion® Spot is a robust spotting buffer that is suitable for use with most
Nexterion® coated slides. The reagent is a modified phosphate buffer with a
pH of 9.0 and is supplied as a 2x concentrated solution. This buffer ensures
excellent spot morphology, high signal intensities, and results in medium to
small spot diameters. In addition, Nexterion® Spot offers users the option of
“tuning” the size of the printed spot by adding varying volumes of detergents.
Specific instructions on how to modify the printing buffer are available
on request, or on the SCHOTT Microarray Solutions website. Nexterion® Spot
is available as a 2x concentration in 100 mL packs.
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Nexterion® Spot A HD
(order code: 1168809)
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Low evaporation DNA spotting buffer for aminosilane coated substrates
For many DNA microarray users, the slide surface of choice is still aminosilane
despite the increasing use of “active” surface chemistries such as epoxysilane.
Although there are many commercially
available spotting buffers already
in use for this slide surface,
many users report issues with
evaporation during long print runs
and spots that are too large for
producing higher density arrays.
To accommodate the needs of
these amino silane slide users,
SCHOTT developed an optimized
spotting buffer for high density
printing applications on Nexterion®
aminosilane coated slides.
Using Nexterion® Spot A HD spotting buffer, spots of 80 µm in diameter can
be printed consistently with a pitch of 130 µm (with pins with a tip diameter
of 50 µm), allowing users to produce up to 50 K arrays with conventional
contact printers. The spotting buffer produces spots with excellent spot morphology
and signal-to-background ratios. In addition to offering smaller spot
sizes for high density arraying, Nexterion® Spot A HD also has impressive low
evaporation properties for longer print runs.
Nexterion® Spot A HD has been optimized for use with Nexterion® Slide A+
and Slide AStar. Nexterion® Spot A HD is available as a 1x concentration in
100 mL packs.
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Nexterion® Spot LE Kit
(order code: 1117902)
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Low evaporation DNA spotting buffer for epoxysilane coated substrates
Higher density arrays usually require extended print runs that typically result
in evaporation from the source plates. To enable long print runs on Nexterion®
Slide E, SCHOTT developed Nexterion® Spot LE, offering users the same performance
features associated with standard Nexterion® Spot, combined with
low evaporation. The spot sizes are typically small enough for higher density
arrays at between 60 – 70 µm, however sarcosyl may be added to increase
spot size if required. The Nexterion® Spot LE protocol provides full details on
the key factors to consider when working with this spotting buffer. SCHOTT
recommends printing with split pins at a maximum pitch of 180 ìm to obtain
20 – 25 K spot arrays. Nexterion® Spot LE kit is available as a 1x concentration
in 100 mL pack with 10 mL sarcosyl solution.
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Nexterion® Spot PB
(order code: 1178050)
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Protein spotting buffer
Nexterion® Spot PB was developed as a protein spotting buffer and has been
optimized to work with all SCHOTT’s dedicated protein slide surfaces, including
Nexterion® Slide P, Slide NC, Slide E and Slide H. It provides outstanding spot
morphologies and contains no BSA, thus eliminating a component that may
compete with low concentration probe samples. Nexterion® Spot PB typically
produces spot sizes between 100 – 160 ìm, depending on the slide surface.
Nexterion® Spot PB is available as a 2x concentration in 100 mL packs.
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Contact
SCHOTT Technical Glass
Solutions GmbH
Otto-Schott-Strasse 13 07745 Jena Germany
 | +49 (0)3641/681-4066 |
 | +49 (0)3641/681-4970 |
E-mail
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