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NEXTERION^® kits

Nexterion® Epoxy Slide Oligo Processing Kit (order code: 1209009) (for 25 slides)

SCHOTT Microarray Solutions offers a kit with pre-prepared reagents for the blocking, hybridization and washing of 25 printed epoxysilane coated slides. The reagents in the kit are optimized for use with epoxy coated slides (e.g. NEXTERION® Slide E) printed with 20 to 70 mer oligonucleotides. The NEXTERION® Oligo Pre-Hyb offers the advantage of combining some of the initial washing and blocking steps thereby reducing the overall processing time. NEXTERION® Oligo Hyb contains formamide, which allows for increased hybridization stringency at decreased hybridization temperatures. The kit includes an easy-to-use slide processing protocol. Superior signal-to-background ratios Enhanced signal-to-background ratios were observed over the entire dynamic range (as shown by the results from low, medium, and high expressor genes) when 70 mer oligonucleotides were spotted onto the NEXTERION® Slide E. The covalent probe binding properties of the slide surface allow the optimal presentation of the probe and therefore improved target accessibility for hybridization. In addition, the reactive groups on the slide surface can be fully deactivated using a blocking reagent, greatly reducing the background fluorescence caused by non-specific binding.

The graph shows normalized signal-to-background ratios (S/Bk) for the market leading aminosilane slide and NEXTERION® Slide E. Signal-to-background ratios were calculated for three selected genes with different expression levels (low, medium and high). To allow the presentation of all the data in one graph, all the results were normalized against the values obtained for the market leading aminosilane slide.

The Epoxy Slide Oligo Processing Kit contains the following components:

Process step Components Reagent concentration Quantity Blocking NEXTERION® Oligo Pre-Hyb Ready-to-use 1x 1 x 500 mL Hybridization NEXTERION® Oligo Hyb Ready-to-use 1x 1 x 10 mL Washing NEXTERION® Wash A 20x 1 x 1000 mL NEXTERION® Oligo Wash B 20x 1 x 100 mL

Protocol

NEXTERION^® Epoxy Slide Oligo Processing Kit

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NEXTERION® Aminosilane Slide Processing Kit (order code: 1209008) (for 25 slides)

SCHOTT Microarray Solutions also offers a kit for 25 printed aminosilane coated slides (e. g. NEXTERION® Slide AStar/A+). The NEXTERION® Block A offers the advantage of combining some of the initial washing and blocking steps thereby reducing the overall processing time. NEXTERION® Oligo Hyb contains formamide, which allows for increased hybridization stringency at decreased hybridization temperatures. The kit includes an easy-to-use slide processing protocol. The Aminosilane Slide Processing Kit contains the following components:

Process step Components Reagent concentration Quantity Blocking NEXTERION® Block A Ready-to-use 1x 1 x 500 mL Hybridization NEXTERION® Oligo Hyb Ready-to-use 1x 1 x 10 mL Washing NEXTERION® Wash A 20x 1 x 1000 mL NEXTERION® Wash B 20x 1 x 100 mL

Protocol

NEXTERION^® Aminosilane Slide Processing Kit

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NEXTERION® “Active” Slide Chemistry Evaluation Kits

To assist customers evaluating the NEXTERION® slide surfaces, SCHOTT offers ready-to-use slide evaluation kits for two of its most commonly used slide surfaces. The NEXTERION® Slide Evaluation Kits offer users the opportunity to fully evaluate the slide performance by utilizing the optimized protocol and ready-to-use reagents included in the kit. The kit consists of ten slides with test oligonucleotides, optimized reagents and detailed protocols.

NEXTERION® Slide E Evaluation Kit (order code: 1117570)

Components Quantity Microarray Slide NEXTERION® Slide E 10 slides Spotting NEXTERION® Spot NEXTERION® Spot LE Kit (Low Evaporation Buffer) 10 mL 10 mL Blocking NEXTERION® Block E 100 mL Hybridization NEXTERION® Hyb 10 mL Test probe Oligo probe 2 nmol Test target Oligo target 3 pmol

NEXTERION® Slide AL Evaluation Kit (order code: 1066026)

Components Quantity Microarray Slide NEXTERION® Slide AL 10 slides Spotting NEXTERION® Spot 10 mL Hybridization NEXTERION® Hyb 10 mL Test probe Oligo probe 2 nmol Test target Oligo target 3 pmol

Download pdf file NEXTERION^® kits