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Multi-well microplate format
NEXTERION® MPX-96 microarraying products
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The microtiter plate format is a standard research tool for clinical diagnostics
and drug discovery. The advantages associated with this format are obvious
and include, high sample throughput, cost savings and compatibility with
automated handling systems. In recent years, there have been efforts to make
the microplate format available for microarraying with the emergence of highresolution
plate scanners and compatible arrayers. However, there are clearly
a number of limitations with the conventional 96-well polymeric and glass
bottom microtiter plates currently being utilized in microarraying, including:
- Microarray print heads cannot access the entire well area because the wells
are recessed. This limits the number of features per well and arrayer compatibility.
- The depth of the wells, and the subsequent additional z-axis travel, makes
printing time consuming.
- The intra-well print area may be restricted due to the contamination of
the well edges with bonding adhesive. In addition, adhesive out gassing
can affect functional coating performance.
- The round wells have a smaller printable area than the square MPX-96 wells.
- Difficulty associated with applying functional coatings to plastic plates.
- Conventional plastic microplates suffer from poor optical transparency and
flatness.
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| Key product features |
| • SBS compilant microplate format |
| • Glass plate easily detaches from the plate holder for spotting
and scanning flexibility |
| • Maximum printable area of 6 mm x 6 mm allows over 1600 features
per well |
| • High optical transmission and low auto fluorescence glass for optimal
performance |
| • Available as a complete microtiter plate, or as individual components with
or without well patterning and/or functional chemistry |
| • Compatible with all contact or non-contact microplate arrayers |
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Product details
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The three main components comprising the NEXTERION® MPX-96 system are available as
a complete kit for evaluation, or as separate items:
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Three components of the NEXTERION® MPX-96 system
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Dimensions NEXTERION® MPX-96 system
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High quality glass substrate
Microarray plate scanners typically read fluorescence signals from the spots
through the bottom of the microplate. The NEXTERION® glass plate
has a high transmittance of over 92 % and exceptional optical clarity for
wavelengths used in fluorescence-based microarray assays. The plates are
manufactured by melting extremely pure raw materials to ensure an excellent
internal glass quality with absence of inclusions, bubbles, streaks or other
defects. The ultra flat, glass plate is manufactured from low auto-fluorescent
borosilicate glass with the dimensions 74 mm x 110 mm x 1 mm. The uniform
flatness of the microplate glass ensures highly reproducible contact
printing and scanning across the wells. The microarray quality glass plate
is available with or without
a 96-well hydrophobic
patterning, and with a
number of functional coatings,
making it suitable for
all microarray applications
using DNA, peptide or
protein probes. The glass
plate may be printed separately
or in the microtiter
plate tray holder.
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Dimensions MPX-96 plate
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96-well silicone superstructure
The optional superstructure is self-adhesive and adheres to the 96-well patterning
on the glass plate, increasing the well volume to between 30 and
130 µL. Placing the adhesive on the superstructure rather than on the glass
plate, avoids the problem of adhesive contamination in the wells.
!! Attention: Please store superstructures flat, and do not bend
as performance may be impaired !!
Microtiter plate holder with lid
The SBS (Society for Biomolecular Screening) compliant rigid polycarbonate
holder supports the glass substrate and superstructure. It also acts as an alignment
jig when attaching the superstructure. The holder has molded alpha/
numeric well labels for easy identification. The four fixing pins firmly hold the
glass plate at the four corners of the microtiter plate holder ensuring the glass
is kept flat. The lid and sealing film limit sample contamination and evaporation
during processing.
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Instrument compatibility
The NEXTERION® MPX-96 plates are compatible with a wide range of commercially available microarray printers, plate washers and scanners.
a) Compatible Microarray printers
b) Compatible Plate Washers
c) Compatible Scanners
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Technical instructions
Further information about printing and processing the MPX-96 plates are
available in the FAQ section.
Packaging and storage
NEXTERION® glass plates are available in single plate kits, or as 5-plate
packs sealed in stable plastic boxes under an inert atmosphere. The plates are
packaged separately to the plastic kit components to avoid any changes to
the coatings caused by outgassing. The exact shelf life of the plates is determined
by the coating and is indicated on the external packaging.
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NEXTERION® A+ MPX-96: DNA application
Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
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NEXTERION® E MPX-96: DNA application
Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
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NEXTERION® E MPX-96: Protein application
Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
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