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Multi-well slide format
Nexterion® MPX-16 Slides
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The special 16-well architecture of Nexterion® Slide MPX allows the parallel
analysis of multiple biological samples against focused subsets of probes on a
single slide. The dimensions of the microarray slide are fully compatible with
all microarray printers and scanners, allowing users to run multiple samples
on a single slide without any additional hardware.
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| Key product features |
| • Standard slide format compatible with all standard commercial micro array
printing, liquid handling and scanning equipment |
| • Simultaneous analysis of 16 biological samples on one slide |
| • Excellent reproducibility compared to conventional single-array
applications |
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Product details
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High quality glass substrate
The ultra flat, glass slide is manufactured from low auto-fluorescent borosilicate
glass with the dimensions 75.6 mm x 25.0 mm x 1 mm. The uniform
flatness of the borosilicate glass ensures highly reproducible contact printing
and scanning across the wells. The glass slides are partitioned into 16 wells
by an ultra-hydrophobic patterning layer. The dimensions and locations of
the wells are fully compatible with standard microarray printing parameters.
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Dimensions of Nexterion® Slide MPX-16.
All dimensions are given in mm.
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16-well silicone superstructure and sealing strip
The optional superstructure is self-adhesive, and adheres to the 16-well
patterning of the glass slide, increasing the well volume to between 30 and
130 µL. Placing the adhesive on the superstructure rather than on the glass
slide, avoids the risk of adhesive contamination in the wells. The clear selfadhesive
sealing strip seals all the individual wells during hybridization preventing
evaporation and cross contamination. If necessary, the superstructure
and sealing strip may be removed prior to scanning.
!! Attention: Please store superstructures flat, and do not bend
as performance may be impaired !!
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MPX-4 four slide microplate adaptor
An SBS microtiter plate sized adaptor, holding up to four MPX, slides is offered
separately. This adaptor allows the use of 8-channel pipettors or microplate
washers, for automated high-throughput processing of Nexterion® Slide MPX.
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Packaging and storage
Nexterion® MPX slides are available as 5-slide packs sealed in stable plastic
boxes under an inert atmosphere. The slides are ready-to-use from the box.
The exact shelf life of the slides is determined by the coating and is indicated
on the external packaging. The 16-well superstructures are not included with
the slides and must be ordered separately.
Accessories
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| Product |
Item number |
Pieces per pack |
| Nexterion® MPX-4 tray |
1180496 |
1 |
| Nexterion® 16-well self-adhesive
superstructure and sealing strip |
1178061 |
5 |
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Design and Printing Guidelines
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Nexterion® Slide MPX provides subarray wells for multiplexed hybridization experiments. The printing of multi-well microarrays vary compared to conventional slide spotting. Due to the special slide architecture, detailed information to support your experimental microarray design are provided. Full design and printing guidelines are to be downloaded below.
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Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
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Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
|
Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
|
Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
|
Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
|
Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
|
Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
|
Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
Date: September 2008
Protocol version: 1.1
Revision made: Changed Tween® concentration (incubation step), pH of blocking solution
Revision reason: Optimization of blocking and incubation
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Protocol revision
Date: April 2009
Protocol version: 1.1
Revision made: New telephone and fax numbers
Revision reason: Update contact information
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Contact
SCHOTT Technical Glass Solutions GmbH
Otto-Schott-Strasse 13 07745 Jena Germany
| +49 (0)3641/681-4066 |
| +49 (0)3641/681-4970 |
E-mail
More contacts
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