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Multi-well slide format
NEXTERION® MPX-16 Slides
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The special 16-well architecture of NEXTERION® Slide MPX allows the parallel
analysis of multiple biological samples against focused subsets of probes on a
single slide. The dimensions of the microarray slide are fully compatible with
all microarray printers and scanners, allowing users to run multiple samples
on a single slide without any additional hardware.
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| Key product features |
| • Standard slide format compatible with all standard commercial micro array
printing, liquid handling and scanning equipment |
| • Simultaneous analysis of 16 biological samples on one slide |
| • Excellent reproducibility compared to conventional single-array
applications |
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Product details
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High quality glass substrate
The ultra flat, glass slide is manufactured from low auto-fluorescent borosilicate
glass with the dimensions 75.6 mm x 25.0 mm x 1 mm. The uniform
flatness of the borosilicate glass ensures highly reproducible contact printing
and scanning across the wells. The glass slides are partitioned into 16 wells
by an ultra-hydrophobic patterning layer. The dimensions and locations of
the wells are fully compatible with standard microarray printing parameters.
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Dimensions of NEXTERION® Slide MPX-16.
All dimensions are given in mm.
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16-well silicone superstructure and sealing strip
The optional superstructure is self-adhesive, and adheres to the 16-well
patterning of the glass slide, increasing the well volume to between 30 and
130 µL. Placing the adhesive on the superstructure rather than on the glass
slide, avoids the risk of adhesive contamination in the wells. The clear selfadhesive
sealing strip seals all the individual wells during hybridization preventing
evaporation and cross contamination. If necessary, the superstructure
and sealing strip may be removed prior to scanning.
!! Attention: Please store superstructures flat, and do not bend
as performance may be impaired !!
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MPX-4 four slide microplate adaptor
An SBS microtiter plate sized adaptor, holding up to four MPX, slides is offered
separately. This adaptor allows the use of 8-channel pipettors or microplate
washers, for automated high-throughput processing of NEXTERION® Slide MPX.
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NEXTERION® IC-16
This is a reusable 16-well incubation chamber consisting of a flexible, black silicon superstructure held within a high quality, black anodized aluminium holder. The silicone superstructure is tightly held against the glass slide surface by two aluminium plates to create a leak-proof seal around each well. The press-fit silicone superstructure eliminates the need for adhesives that are autofluorescent, and can increase background signal of the slide coating. The superstructure forms sixteen, 7 by 7 mm wells with 9 mm center-to-center spacing, in a 2 x 8 format (identified as 1-2 and A–H). It is ideal for use with multi-channel pipettes or automated liquid handling systems. The chamber is designed to be re-usable, replaceable components are available to maximise the usable life.
Note: The 16-well superstructure is asymmetric, so for the correct orientation, ensure that the wider well wall side of the superstructure is fitted into the upper plate, and the thinner portion is against the glass slide surface.
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Packaging and storage
NEXTERION® MPX slides are available as 5-slide packs sealed in stable plastic
boxes under an inert atmosphere. The slides are ready-to-use from the box.
The exact shelf life of the slides is determined by the coating and is indicated
on the external packaging. The 16-well superstructures are not included with
the slides and must be ordered separately.
Accessories
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| Product |
Item number |
Pieces per pack |
| NEXTERION® MPX-4 tray |
1180496 |
1 |
| NEXTERION® 16-well self-adhesive
superstructure and sealing strip |
1178061 |
5 |
NEXTERION® IC-16
1 x Re-usable 16-well Incubation chamber consisting of lower tray (in black aluminium), upper plate (in black aluminium), 16-well black silicone superstructure, and four corner screw fasteners. |
1262705 |
1 |
NEXTERION® IC-16s
16-well black silicon superstructure for use in the NEXTERION® IC-16 incubation chamber
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1262706 |
1 |
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Design and Printing Guidelines
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NEXTERION® Slide MPX provides subarray wells for multiplexed hybridization experiments. The printing of multi-well microarrays vary compared to conventional slide spotting. Due to the special slide architecture, detailed information to support your experimental microarray design are provided. Full design and printing guidelines are to be downloaded below.
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Printing Guideline NEXTERION® Slide MPX 16
NEXTERION® Slide AStar MPX 16: DNA application
Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
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NEXTERION® Slide A+ MPX 16: DNA application
Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
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NEXTERION® Slide AL MPX 16: DNA application
Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
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NEXTERION® Slide E MPX 16: DNA application
Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
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NEXTERION® Slide H MPX 16: DNA application
Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
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NEXTERION® Slide AL MPX 16: Protein application
Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
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Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
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NEXTERION® Slide H MPX 16: Protein application
Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
Date: September 2008
Protocol version: 1.1
Revision made: Changed Tween® concentration (incubation step), pH of blocking solution
Revision reason: Optimization of blocking and incubation
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