Three-dimensional polymer coating
The coating on the SCHOTT NEXTERION® Slide P is a three-dimensional, hydrophilic polymer activated with N-Hydroxysuccinimide (NHS) esters to provide covalent immobilisation of amine groups. All NEXTERION® microarray slides are manufactured from a high quality, low-fluorescence glass coated with low-fluorescence coatings. However the non-specific binding of assay components, especially from serum samples, still remains an important contributor to the off feature background for many microarray applications. For most types of slide coatings the post-print processing protocol involves a method of adsorptive blocking to reduce non-specific binding; however these procedures are difficult to perform in a consistent manner.
The NEXTERION® Slide P coating has been engineered to exhibit an extremely low intrinsic non-specific background without the need for blocking. This was achieved by using a special polymer that is extremely resistant to non-specific binding. During in-house tests run by SCHOTT, NEXTERION® Slide P had the lowest background signal of any microarray slide coating ever tested. The polymer coating has a three-dimensional structure; with NHS-ester reactive groups attached to the hydrophilic polymer layer. The surface-exposed amino-groups on the antibodies react immediately and irreversibly with the NHS-ester groups forming covalent bonds. The polymer coating maintains the immobilised antibodies in a quasi-liquid environment that maintains their functionality and specificity. The polymer, combined with the end-point attachment, orients the immobilized bio-molecules away from the glass facilitating the interactions of the attached antibodies with the target antigens in the assay sample.
Highly reproducible coating
NEXTERION® Slide P is fabricated using a proprietary deposition process developed by SCHOTT to produce a uniform and reproducible polymer coating on one side of a high quality borosilicate glass slide. All slides are individually examined for physical defects and the absence of particle contamination before and after coating. The coating is applied in a tightly controlled, Class 100 clean room facility, resulting in coated slides with highly uniform surface properties and low auto-fluorescence.
Excellent spot morphology and signal-to-background ratios
NEXTERION® Slide P provides excellent spot morphologies and reproducible spot sizes over a wide range of probe concentrations.
Figure shows scanned images of NEXTERION® Slide P evaluated in an anti-IgG/IgG interaction study over a range of probe concentrations dissolved in NEXTERION® Spot PB and a phosphate print buffer.
The NEXTERION® Slide P coating is relatively hydrophilic, and care must be taken to ensure that the spot-to-spot pitch distance is set high enough to avoid spot merging.
Packaging and storage
NEXTERION® Slide P are packaged in chemically stable plastic boxes and sealed under an inert atmosphere. The slides are ready-to-use from the box, and are stable for up to 12 months in the sealed packaging when stored at –20ºC. .
NEXTERION® Slide P is available in packs of 25-slides with Code 128 barcodes enabling automated sample tracking. The identical P coating is also available in either 16-well, 48-well or 96-well formats. For further information refer to the section “Multi-well formats”.
NEXTERION® Slide P: Protein application
Protocol revisionMSDS NEXTERION® Slide P Download pdf file 'Nexterion® Slide P'
Date: January 2011
Protocol version: 1.3
Revision made: Protocol optimized for printing and processing antibody arrays
Revision reason: To make protocol application specific
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
Date: August 2008
Protocol version: 1.1
Revision made: Changed Tween® concentration (incubation step), pH of blocking solution
Revision reason: Optimization of blocking and incubation
SCHOTT Technical Glass Solutions GmbH