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Epoxysilane coating
Nexterion® Slide E
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Nexterion® Slide E is the market leading epoxysilane coated slide from
SCHOTT. The slides are manufactured from high quality borosilicate glass
that has an ultra flat surface and low inherent fluorescence. The glass is
coated with a multi-purpose epoxysilane layer that will covalently bind most
types of bio-molecules including amino- and non-modified DNA, RNA, and
proteins. The defect-free surface features a uniform epoxysilane layer that
provides a high covalent coupling efficiency together with a very low background.
The slides are easy to use, and are fully compatible with all commercially
available arraying and scanning instruments.
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| Type of
coating |
Immobilization
method |
Typical
probes |
Ordering information |
| Nexterion® product |
Barcode
option |
Item
number |
Slides
per pack |
| Epoxysilane
2-D surface |
Amino-, thiol- and hydroxylreactive
chemistry
Covalent binding |
• Amino-modified or
unmodified oligos,
mRNA, PCR and BACs
• Proteins |
Slide E |
None |
1066643 |
25 |
| Laser |
1064016 |
25 |
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| Key product features |
| • Covalent and directed binding of DNA and proteins |
| • Multi-purpose microarray slide coating |
| • Hydrophobic coating enables the spotting of high-density arrays
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| • Stability of the epoxy chemistry supports long print runs |
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| Typical applications |
| • Transcriptional profiling |
| • MicroRNA |
| • Array-CGH |
| • SNP detection |
| • ZIP-code universal microarray |
| • Pathogen identification |
| • Subtelomere arrays |
| • Functional protein arrays |
| • Protein profiling arrays |
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| Suitable probe types |
| • Amino-modified oligonucleotides 20 – 70 mers |
| • Unmodified oligonucleotides 20 – 70 mers |
| • Amino-modified PCR products |
| • ZIP-code oligonucleotides |
| • PCR products |
| • BAC/PACs |
| • L-DNA |
| • cDNA |
| • RNA |
| • Serum samples |
| • Antibodies |
| • Peptides |
| • Glycans |
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The diagram shows a schematic representation of an amino-modified DNA
molecule coupling to Nexterion® Slide E. Amino-modified nucleic acids bind
via the terminal amino group, as well as via exocyclic amino groups of the
bases, and hydroxyl groups of the sugar-phosphate backbone. Unmodified
nucleic acids will bind to an epoxysilane surface with almost the same efficiency
via the exocyclic amino groups of the bases, and hydroxyl groups of
the backbone. Other bio-molecules such as proteins bind via their exposed
amine-, thiol- and hydroxyl-groups.
The reaction with the epoxy group is
rapid and irreversible and no additional
baking or UV cross-linking steps are
required. Any unreacted epoxy group
may be chemically deactivated after
printing to significantly reduce nonspecific
binding of the target molecules
to the slide surface. Molecular spacers
between the glass slide and the epoxy
groups facilitate interactions between
the printed bio-molecules and their
binding partners in solution.
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Product details
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Highly reproducible coating
Nexterion® Slide E is produced using an innovative proprietary process developed
by SCHOTT to produce a uniform and reproducible epoxysilane coating
on both sides of a high quality borosilicate glass slide. All slides are individually
examined for physical defects and the presence of particles before and
after coating. The epoxysilane surface is applied in tightly controlled, class
100 clean room facility, resulting in coated slides with highly uniform surface
properties and low auto-fluorescence.
Fully optimized surface
Nexterion® Slide E provides excellent spot morphologies and reproducible
spot sizes with a variety of commonly used spotting buffers. The surface
chemistry is robust and stable, and remains active even during very long print
runs. The density of the epoxy groups in the coating is constant over the
entire surface of the slides, and has been optimized to provide uniform spot
size and shape and the optimal binding capacity. Another important parameter,
surface hydrophobicity, is also tightly controlled to optimize the
performance of the slides with both contact and non-contact printers.
Packaging and storage
Nexterion® Slide E are packaged in chemically stable plastic boxes and sealed
under an inert atmosphere. The slides are ready-to-use from the box, and
are stable for 12 months in the sealed packaging when stored at room temperature.
Format
The slides are available in packs of 25 slides with optional code 128 barcodes
enabling automated sample tracking. The identical epoxysilane coating is also
available in 16-well slide and 96-well microplate multi-well formats. For further
information refer to the section on “Multi-well formats”.
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| Protocol step |
Recommended
Nexterion® products |
Alternatives |
Remarks |
| Spotting |
Nexterion® Spot
Nexterion® Spot LE Kit
(low evaporation buffer) |
Nexterion® Spot + detergents like
Cetyltrimethylammoniumbromide,
Triton X-100, sarcosyl, Tween
or SDS with a final concentration
between 0.005 and 0.05 %
(to increase the spot size)
3x SSC |
Recommended Concentrations:
Oligonucleotides: 10 – 20 µM
PCR products: 0.1 – 0.5 µg/µL |
| Chemical deactivation
of unreacted
Epoxy groups |
Nexterion® Block E
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50 mM ethanolamine + 0.1 % SDS
in 0.1 M Tris, pH 9.0 |
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| Nexterion® Oligo Pre-Hyb |
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| Hybridization |
Nexterion® Hyb |
3 – 5x SSC + 0.1 % SDS |
No formamide |
| Nexterion® Oligo Hyb |
|
Contains formamide |
| Washing steps |
Nexterion® Wash A and B |
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Nexterion® Slide E Evaluation Kit
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Nexterion® Slide E is easy-to-use, but as this type of slide chemistry may be
less familiar to some researchers, a dedicated Nexterion® Slide E Evaluation Kit
is available. The kit consists of slides with test oligonucleotides and optimized
reagents, allowing new users to carry out a test print and hybridization.
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| Process step |
SCHOTT product |
Quantity |
| Spotting |
Nexterion® Slide E |
10 slides |
Nexterion® Spot
Nexterion® Spot LE Kit
(Low Evaporation Buffer) |
10 mL
10 mL |
| Blocking |
Nexterion® Block E |
100 mL |
| Hybridization |
Nexterion® Hyb |
10 mL |
| Oligo test probe |
2 nmol |
| Oligo test target |
3 pmol |
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Nexterion® Epoxy Slide Oligo
Processing Kit
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SCHOTT Microarray Solutions offers a kit with pre-prepared reagents for the
chemical deactivation, hybridization and washing of 25 printed epoxysilane
coated slides. The reagents in the kit are optimized for use with slides printed
with 20 to 70 mers oligonucleotides.
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| Process step |
SCHOTT product |
Quantity |
| Blocking |
Nexterion® Oligo Pre-Hyb |
500 mL |
| Hybridization |
Nexterion® Oligo Hyb |
10 mL |
| Washing |
Nexterion® Oligo Wash A |
1000 mL |
| Nexterion® Oligo Wash B |
100 mL |
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Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
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Protocol revision
Date: April 2009
Protocol version: 1.1
Revision made: New telephone and fax numbers
Revision reason: Update contact information
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Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information
|
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Contact
SCHOTT Technical Glass
Solutions GmbH
Otto-Schott-Strasse 13 07745 Jena Germany
 | +49 (0)3641/681-4066 |
 | +49 (0)3641/681-4970 |
E-mail
More contacts
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