Epoxysilane coating


NEXTERION® Slide E

NEXTERION® Slide E is the market leading epoxy coated slide from SCHOTT. The slides are manufactured from high quality borosilicate glass that has an ultra flat surface and low inherent fluorescence. The glass is coated with a multi-purpose epoxysilane layer that will covalently bind most types of bio-molecules including amino- and non-modified DNA, RNA, and proteins. The defect-free surface features a uniform epoxysilane layer that provides a high covalent coupling efficiency together with a very low background. The slides are easy to use, and are fully compatible with all commercially available arraying and scanning instruments.


Overview

Type of coating Immobilization method Typical probes Ordering information
NEXTERION® product Barcode option Item number Slides per pack
Epoxysilane 2-D surface Amino-, thiol- and hydroxylreactive chemistry Covalent binding • Amino-modified or unmodified oligos, mRNA, PCR and BACs
• Proteins
Slide E None 1066643 25
Laser 1064016 25
Key product features
• Covalent and directed binding of DNA and proteins
• Multi-purpose microarray slide coating
• Hydrophobic coating enables the spotting of high-density arrays
• Stability of the epoxy chemistry supports long print runs
Typical applications
• Transcriptional profiling
• MicroRNA
• Array-CGH
• SNP detection
• ZIP-code universal microarray
• Pathogen identification
• Subtelomere arrays
• Functional protein arrays
• Protein profiling arrays
Suitable probe types
• Amino-modified oligonucleotides 20 – 70 mers
• Unmodified oligonucleotides 20 – 70 mers
• Amino-modified PCR products
• ZIP-code oligonucleotides
• PCR products
• BAC/PACs
• L-DNA
• cDNA
• RNA
• Serum samples
• Antibodies
• Peptides
• Glycans

Immobilization chemistry

Coating chemistry of NEXTERION epoxysilane coated microarray slides


The diagram shows a schematic representation of an amino-modified DNA molecule coupling to NEXTERION® Slide E. Amino-modified nucleic acids bind via the terminal amino group, as well as via exocyclic amino groups of the bases, and hydroxyl groups of the sugar-phosphate backbone. Unmodified nucleic acids will bind to an epoxysilane surface with almost the same efficiency via the exocyclic amino groups of the bases, and hydroxyl groups of the backbone. Other bio-molecules such as proteins bind via their exposed amine-, thiol- and hydroxyl-groups. The reaction with the epoxy group is rapid and irreversible and no additional baking or UV cross-linking steps are required. Any unreacted epoxy group may be chemically deactivated after printing to significantly reduce nonspecific binding of the target molecules to the slide surface. Molecular spacers between the glass slide and the epoxy groups facilitate interactions between the printed bio-molecules and their binding partners in solution.


Product details

Highly reproducible coating
NEXTERION® Slide E is produced using an innovative proprietary process developed by SCHOTT to produce a uniform and reproducible epoxysilane coating on both sides of a high quality borosilicate glass slide. All slides are individually examined for physical defects and the presence of particles before and after coating. The epoxysilane surface is applied in tightly controlled, class 100 clean room facility, resulting in coated slides with highly uniform surface properties and low auto-fluorescence.

Fully optimized surface
NEXTERION® Slide E provides excellent spot morphologies and reproducible spot sizes with a variety of commonly used spotting buffers. The surface chemistry is robust and stable, and remains active even during very long print runs. The density of the epoxy groups in the coating is constant over the entire surface of the slides, and has been optimized to provide uniform spot size and shape and the optimal binding capacity. Another important parameter, surface hydrophobicity, is also tightly controlled to optimize the performance of the slides with both contact and non-contact printers.

Packaging and storage
NEXTERION® Slide E are packaged in chemically stable plastic boxes and sealed under an inert atmosphere. The slides are ready-to-use from the box, and are stable for 12 months in the sealed packaging when stored at room temperature.

Format
The slides are available in packs of 25 slides with optional code 128 barcodes enabling automated sample tracking. The identical epoxysilane coating is also available in 16-well slide, 48-well slide and 96-well microplate multi-well formats. For further information refer to the section on “Multi-well formats”.

Compatible reagents for printing DNA and Oligos 

Protocol
step
Recommended NEXTERION® products Alternatives Remarks
Spotting NEXTERION® Spot
(1066029)
NEXTERION® Spot + detergents like Cetyltrimethylammoniumbromide, Triton X-100, sarcosyl, Tween or SDS with a final concentration between 0.005 and 0.05 % (to increase the spot size)

3x SSC


NEXTERION® Spot LE
(low evaporation buffer)
(composition can be provided)

Recommended Concentrations:
Oligonucleotides: 10 – 20 µM
PCR products: 0.1 – 0.5 µg/µL
Chemical deactivation of unreacted Epoxy groups NEXTERION® Block E
(1066071)
50 mM ethanolamine + 0.1 % SDS in 0.1 M Tris, pH 9.0  
  NEXTERION® Oligo Pre-Hyb
(composition can be provided)
 
Hybridization NEXTERION® Hyb
(1066075)
3 – 5x SSC + 0.1 % SDS No formamide
  NEXTERION® Oligo Hyb 
(composition can be provided)
Contains formamide
Washing steps - NEXTERION® Wash A and B
(composition can be provided)
 

Protocols

Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information

Date: September 2010
Protocol version: 1.3
Revision made: recommend additional incubation step after humidity chamber (4.2)
Revision reason: better performance
Protocol revision
Date: April 2009
Protocol version: 1.1
Revision made: New telephone and fax numbers
Revision reason: Update contact information



Material Safety Datasheets

Trade Fairs & Events
21.
March
Trade fair Workshop "Microarrays: Basics, Production and Processing", ETH Zurich / University of Zurich, Switzerland, 21.03 - 22.03.2017