Aldehydesilane coating


NEXTERION® Slide AL

NEXTERION® Slide AL offers a good alternative to NEXTERION® Slide E (Epoxysilane slides) when an efficient covalent, and directed binding of amino-modified bio-molecules is required. NEXTERION® Slide AL is coated with an “active” slide chemistry coating that chemically binds bio-molecules to provide a high binding capacity, and minimize non-specific binding. The uniform surface features aldehyde groups that readily react with primary amines. Both 5’ or 3’ amine modified PCR products, and oligonucleotides work well with this surface chemistry. The covalent bond formed at the terminus of the nucleic acid offers both stability, and maximal base pairing opportunity. The covalent binding diminishes sample loss during the course of experiments, and permits more harsh wash steps, which reduce background, and allow for greater sensitivity. Additional immobilization steps, such as baking or UV cross-linking, are not required for immobilization. Furthermore, peptides, proteins (such as antibodies), cells, and tissues can be immobilized on NEXTERION® Slide AL via random binding to amine sites on the probes. The slides are easy to use, and are compatible with all commercially available arraying and scanning instruments.


Overview

Type of coating Immobilization method Typical probes Ordering information
NEXTERION® product Barcode option Item number Slides per pack
Aldehydesilane 2-D surface Amine reactive chemistry
Covalent binding
Amino-modified PCR products, BACs and oligonucleotides Slide AL None 1064874 25
Laser 1064876 25
Key product features
• Stable and covalent binding of probes such as amino-modified nucleic acids, cells, and peptides
• Optimal accessibility of probes through specific end-point attachment
Typical applications
• Array CGH using whole genome tiling path BAC arrays
• miRNA expression profiling
• Indel oligonucleotide arrays for genotyping
• 16S rRNA-based taxonomic microarray
• Protein-DNA interaction studies
• Protein-protein interaction studies
Suitable probe types
• Amine-PCR products and BACs
• Amino-modified oligonucleotides
• Small protein fragments such as peptides
• Cells and tissues

Immobilization chemistry




The DNA product is spotted onto the aldehydesilane slide. The primary amino linkers (NH2) on the DNA attack the aldehyde groups to form covalent bonds (Schiff’s base). The attachment is stabilized by a dehydration reaction. To minimize fluorescent background, unreacted aldehyde groups are reduced to non-reactive primary alcohols by treatment with sodium borohydride. In addition, this step reduces the double bond between the probe and surface, producing an irreversible covalent immobilization. Additional steps for immobilization, such as baking, or UV cross-linking, are not required.


Product details

Highly reproducible coating

NEXTERION® Slide AL are produced using an innovative process developed by SCHOTT to produce a consistent and reproducible coating. All slides are individually examined for physical defects and the presence of particles before and after coating. The density of the aldehyde groups on the coating remain constant over the entire surface of the slides, and has been optimized to provide the optimal binding capacity. The surface hydrophobicity is tightly controlled to optimize the performance with both contact and non-contact printers.
High intra-slide reproducibility of NEXTERION® Slide AL (20 k array of BSA labelled with Atto 547)
Packaging and storage

NEXTERION® Slide AL are packaged in chemically stable plastic boxes and sealed in an inert atmosphere. The slides are ready-to-use from the box, and are stable for 9 months in the sealed packaging when stored at room temperature.

Format

The slides are available in packs of 25-slides with optional code 128 barcodes enabling automated sample tracking. The aldehydesilane coating is also available in 16-well slide, 48-well slide and 96-well microplate formats. For further information refer to the section on “Multi-well formats”.

Compatible reagents

Protocol
step
Recommended NEXTERION® products Alternatives Additional information
Spotting NEXTERION® Spot 
(1066029)
NEXTERION® Spot + detergents like cetyltrimethylammoniumbromid, Triton X-100, sarcosyl, Tween or SDS with a final concentration between 0.005 and 0.05 % (to increase the spot size) Recommended Spotting Concentrations:
PCR products: 0.1 – 1 µg/µL
Oligonucleotides: 10 – 20 µM
Peptides: 100 – 500 µg/mL
Recommended Spotting Conditions:
Constant relative humidity 40 – 50% at 20 – 24°C (68 to 75°F). Spotting solutions may be used with, or without protein-stabilizing agents
3x SSC
3x SSC + 1.5 M betaine (low evaporation buffer for long spotting runs)
Chemical deactivation - 1.0 g NaBH4 in 300 mL PBS and 100 mL ethanol  
Hybridization NEXTERION® Hyb (formamide free)
(1066075)
3 – 5x SSC + 0.1 % SDS Add competitor DNA if appropriate

Protocols

Protocol revision
Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: correction contact information
Protocol revision
Date: April 2009
Protocol version: 1.1
Revision made: New telephone and fax numbers
Revision reason: correction contact information



Material Safety Datasheets



Publications citing NEXTERION® Slide AL


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Trade Fairs & Events
21.
March
Trade fair Workshop "Microarrays: Basics, Production and Processing", ETH Zurich / University of Zurich, Switzerland, 21.03 - 22.03.2017