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Frequently Asked Questions (FAQ)



Welcome to the frequently asked questions (FAQ) section. This list is designed to assist in answering questions related to Nexterion® microarray products.

The list is divided into the following sections:

  • General questions about Nexterion® products
  • Questions related to the microarray process
  • Microarraying on microtiter plate format

  • Image gallery of typical microarray problems

     If your microarray spots don't appear as they should, have a look at the image gallery of typical problems.
    Link to the gallery



    • General questions about Nexterion® Products








    Top
    Questions around Microarraying

    Printing and Immobilization
    The printing process accomplishes the delivery of the probes onto the microarray slide and should be as uniform as possible to allow a reliable data generation. During immobilization probes are covalently attached to the surface to allow for subsequent processing.



    Top
    Blocking
    Blocking is conducted after the immobilization of the probes, and either inactivates the remaining active groups on the surface, or blocks the surface by complete occupation of the remaining binding sites.
    Hybridization
    The hybridization process should be designed to allow the specific matching of the labeled binding partner, but at the same time avoiding unspecific binding of non-complementary sequences. Moreover, unspecific binding of any other fluorescent compounds to the surface should be minimized to maximise the sensitivity of the experiment.


    Scanning
    Microarraying on microtiter plate format
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