FAQ - Aminosilane coated Slides
Can SCHOTT recommend any blocking protocols for the NEXTERION® aminosilane slides that do not involve BSA?
Yes, it is possible to chemically block the surface of NEXTERION® Slide A+ and Astar. Unfortunately in most cases, the results are inferior to the BSA blocking method.
For this reason, we removed the method from our standard protocols some time ago. However, if you wish to try it, here are details of the chemical blocking for NEXTERION® A+/AStar, it should take 20 minutes:
Amino Blocking Solution: 5 g succinic anhydride + 315 ml n-methylpyrrolidone + 35 ml 0.2 M sodium-borate solution pH 8. Add sodium borate freshly before use.
For printing DNA oligonucleotides onto NEXTERION® aminosilane slides (Slides A / A+ / A*), your protocols state that I can use 50% DMSO. What should I dilute the DMSO with?
If your oligos are already in a buffer, the most usual procedure is to mix them with DMSO and ddH2O to a final concentration of 1×print buffer / 50% DMSO.
SCHOTT Technical Glass Solutions GmbH